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GLaDOS (Genetic Lifeform and Disk Operating System) is the central core designed to control, guide, and oversee the Aperture Science computer-aided Enrichment Center. With the ability to shift and move facilities and various chambers, GLaDOS is able to achieve a seamless and almost infinite testing design whilst residing in the Central AI Chamber.

In possession of a feminine programming, she is the antagonist of Portal and the first half of the single-player campaign in Portal 2. During the later half, she becomes the game's tritagonist. In the game's cooperative campaign, GLaDOS is the testing supervisor of Atlas and P-body.

wiki[theportalwiki.com]