If you are using a standard introduction spawn, like the truck or helicopter, I think it activates an Area(0,0) trigger. So it is probably best to delay the activation of the first trigger in the beginning also, or just use a SpawnPoint doodad.

thanks for your comment. you gave me a much better idea for how to do rescue bros chapter 2

Just so you know:
An Area(0,0) trigger is equivalent to a "Rescue a Bro" trigger, because the new bro is loaded up at the origin point. So if you make a map like this again, try stacking a bunch of Area triggers at (0,0). The first trigger will be initially activated while the others are not. The second trigger is activated by a short delay (2 seconds) after the first bro is saved. Then the third will be activated by a short delay after the second bro saved and so on. The short delay is needed as it might double count a single rescue.

other things:
-Try not to make simultaneous rescues possible when using this, as some might not count.
-Put a steel block on (0,0) to avoid illegal triggers.

Thanks!

Brilliant level desing, keep doing good work. :)