PewDiePie
Will be available at the beginning of the game.

Marzia

Will be available to purchase after the level "Pewd's House".

Markiplier

Will be available to purchase after the level "Jet Fighter".

CinnamonToastKen

Will be available to purchase after the level "Generoll Barrell".

JackSepticEye

Will be available to purchase after the level "Deep in the Mines".

Cryaotic

Will be available to purchase after the level "UFO Computer".

Duck

Will be available to purchase after collecting all the patches.
He costs 2500 Bro Coins.